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KMID : 1005220150300010001
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Journal of Embryo Transfer
2015 Volume.30 No. 1 p.1 ~ p.6
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Evaluation of Toxicity of Green Tea Extract in Chilled Boar Spermatozoa
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Park Sang-Hyoun
Yu Il-Jeoung
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Abstract
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The cold shock of spermatozoa is associated with oxidative stress induced by reactive oxygen species. This study was conducted to evaluate the toxicity of natural antioxidant green tea extract (GTE) in lactose-egg yolk (LEY) extender during boar sperm cooling prior to freezing. Spermatozoa were cooled to 5℃ for 3 h in LEY extender containing 0 (control), 1, 10, 100 or 1,000 mg/l of GTE, re-suspended with LEY-glycerol-Equex extender and cooled at 5℃ for 30 min. Sperm progressive motility, viability and phosphatidylserine (PS) translocation were evaluated. PS translocation was assayed by flow cytometry using Annexin V-FITC apoptosis detection kit. The sperm function including progressive motility, viability and PS translocation was not significantly different regardless of GTE concentrations (P>0.05). In conclusion, this study demonstrated non-toxicity of GTE supplement in LEY extender during sperm cooling.
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KEYWORD
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boar , spermatozoa , green tea extract , cooling , PS translocation
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